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Assay and Cytometry Methodologies

High-throughput assays of phagocytosis, phagosome maturation and bacterial invasion. Steinberg BE, Scott CC, Grinstein S.; Am J Physiol Cell Physiol. 2006 Oct 4;. PMID: 17020932 [PubMed - Publisher]

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Articles by Antigen

Escherichia coli

Ahmed A, Li J, Shiloach Y, Robbins JB, Szu SC  Safety and Immunogenicity of Escherichia coli O157 O-Specific Polysaccharide Conjugate Vaccine in 2-5-Year-Old Children. J Infect Dis. 2006 Feb 15;193(4):515-21. Epub 2006 Jan 13. PMID: 16425130 [PubMed - In-Data-Review]

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Group B Streptococcus

Vaccination against Group B streptococcus. Vaccination against Group B streptococcus. Heath PT, Feldman RG. Department of Child Health and Vaccine Institute, St George's Hospital Medical School, ..

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Neisseria meningitidis

Opsonophagocytosis of Fluorescent Polystyrene Beads Coupled to Neisseria meningitidis Serogroup A, C, Y, or W135 Polysaccharide Correlates with Serum Bactericidal Activity  Journal: Clinical and Diagnostic Laboratory Immunology ISSN/ISBN: 1071-412X  Source: Vol 9 Iss 2 Page: 485-488

Functional opsonic activity of human serum antibodies to inner core lipopolysaccharide (galE) of serogroup B meningococci measured by flow cytometry. Plested JS, Ferry BL, Coull PA, Makepeace K, Lehmann AK, MacKinnon FG, Griffiths HG, Herbert MA, Richards JC, Moxon ER.Infect Immun. 2001 May;69(5):3203-13

Predictors of Immunity after a Major Serogroup W-135 Meningococcal Disease Epidemic, Burkina Faso, PL Raghunathan, JD Jones, SR Tiendrebéogo, I … - The Journal of Infectious Diseases, 2006 - journals.uchicago.edu ... 0022-1899/2006/19305-0001$15.00. MAJOR ARTICLE. Predictors of Immunity after a Major Serogroup W-135 Meningococcal Disease Epidemic, Burkina Faso, 2002. ...

Characterization of the Key Antigenic Components and Pre-Clinical Immune Responses to a Meningococcal Disease Vaccine Based on Neisseria lactamica Outer Membrane Vesicles, Finney M, Vaughan T, Taylor S, Hudson MJ, Pratt C, Wheeler JX, Vipond C, Feavers I, Jones C, Findlow J, Borrow R, Gorringe A - Hum Vaccin. 2007 Jul 29;3(5). PMID: 17921703 [PubMed - Publisher]

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Staphylococcus Aureus

Inhibition of complement activation by a secreted Staphylococcus aureus protein. 
Lee LY, Hook M, Haviland D, Wetsel RA, Yonter EO, Syribeys P, Vernachio J, Brown EL.  J Infect Dis. 2004 Aug 1;190(3):571-9. Epub 2004 Jul 06

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Streptococcus pneumoniae

Evaluation of Multiplex Flow Cytometric Opsonophagocytic Assays for Determination of Functional Anticapsular Antibodies to Streptococcus pneumoniae. Joseph E. Martinez, Elizabeth A. Clutterbuck, Han Li, Sandra Romero-Steiner, and George M. Carlone, Clin. Vaccine Immunol. 2006 13: 459-466. [Abstract] [Full Text] [PDF]

Use of Opsonophagocytosis for Serological Evaluation of Pneumococcal Vaccines. Sandra Romero-Steiner,1 Carl E. Frasch,2 George Carlone,1 Roland A. Fleck,3Baxendale HE, Goldblatt D  ...cvi.asm.org/cgi/content/full/13/2/165

Effect of opsonophagocytosis mediated by specific antibodies on the co-amoxiclav serum bactericidal activity against Streptococcus pneumoniae ...
jac.oupjournals.org/cgi/content/abstract/55/5/742 - Similar pages

Development of a Multiplexed Bead-based Flow Cytometric, Opsonophagocytic Assay for the Simultaneous Detection of Antibody to Multiple Streptococcus pneumoniae Serotypes. (pdf)  J.E. Martines, E. Clutterbuck, S. Romero-Steiner, L.L. Wood, and G.M. Carlone, Centers for Disease Control and Prevention, Atlanta, GA and Flow Applications, Inc, Okawville, IL

Perfomance of a Multiplexed Streptococcus pneumoniae  Flow Cytometric Opsonophagocytic Assay (pdf).  J.E. Martinez, E. Clutterbuck, S. Romero-Steiner, and G.M. Carlone, Centers for Disease Control and prevention, Atlanta, GA

Multilaboratory evaluation of a viability assay for measurement of opsonophagocytic antibodies specific to the capsular polysaccharides of Streptococcus pneumoniae.  Romero-Steiner S, Frasch C, Concepcion N, Goldblatt D, Kayhty H, Vakevainen M, Laferriere C, Wauters D, Nahm MH, Schinsky MF, Plikaytis BD, Carlone GM. Clin Diagn Lab Immunol. 2003 Nov;10(6):1019-24.

Correlates of immunity for pneumococcal conjugate vaccines.  Lee LH, Frasch CE, Falk LA, Klein DL, Deal CD.  Vaccine. 2003 May 16;21(17-18):2199-205.

Randomized, Double-Blind, Controlled Trial of Pneumococcal Vaccination in Renal Transplant Recipients. Kumar D, Rotstein C, Miyata G, Arlen D, Humar A. J Infect Dis. 2003 May 15;187(10):1639-45. Epub 2003 Apr 23.

Are the opsonophagocytic activities of antibodies in infant sera measured by different pneumococcal phagocytosis assays comparable?  Vakevainen M, Jansen W, Saeland E, Jonsdottir I, Snippe H, Verheul A, Kayhty H.Clin Diagn Lab Immunol. 2001 Mar;8(2):363-9.

Functional activity of antibodies after immunization of Finnish and Israeli infants with an 11-valent pneumococcal conjugate vaccine. Wuorimaa TK, Dagan R, Bailleux F, Haikala R, Ekstrom N, Eskola J, Yaich M, Kayhty H Vaccine 2005 Aug 20;.
PMID: 16118028 [PubMed - Publisher] RelatedArticles

A flow cytometric opsonophagocytic assay for measurement of functional antibodies elicited after vaccination with the 23-valent pneumococcal polysaccharide vaccine.  Martinez JE, Romero-Steiner S, Pilishvili T, Barnard S, Schinsky J, Goldblatt D, Carlone GM.Clin Diagn Lab Immunol. 1999 Jul;6(4):581-6.

Serological criteria for evaluation and licensure of new pneumococcal conjugate vaccine formulations for use in infants.  Jodar L, Butler J, Carlone G, Dagan R, Goldblatt D, Kayhty H, Klugman K, Plikaytis B, Siber G, Kohberger R, Chang I, Cherian TVaccine. 2003 Jul 4;21(23):3265-72.

Comparison of an opsonophagocytic assay and IgG ELISA to assess responses to pneumococcal polysaccharide and pneumococcal conjugate vaccines in children and young adults with sickle cell disease.  Vernacchio L, Romero-Steiner S, Martinez JE, MacDonald K, Barnard S, Pilishvili T, Carlone GM, Ambrosino DM, Molrine DC.  J Infect Dis. 2000 Mar;181(3):1162-6.

Immunogenicity, Safety, and Predictors of Response After a Pneumococcal Conjugate and Pneumococcal Polysaccharide Vaccine Series in Human Immunodeficiency Virus-Infected Children Receiving Highly Active Antiretroviral Therapy. Abzug MJ, Pelton SI, Song LY, Fenton T, Levin MJ, Nachman SA, Borkowsky W, Rosenblatt HM, Marcinak JF, Dieudonne A, Abrams EJ, Pathak I Pediatr Infect Dis J. 2006 Oct;25(10):920-929.
PMID: 17006288 [PubMed - Publisher]

European Society of Clinical Microbiology and Infectious Diseases Tabaraiee B. -, A flow cytometric opsonophagocytic assay for measurement of ... Talbott J. -, Performance evaluation of the Streptococcus pneumoniae ...

Streptococcus pneumoniae capsular polysaccharides in human serum and their. correlation to opsonophagocytic assay. DT.08. Pavliakova, D, Belanger, TJ, Kwok, ...

Wuorimaa TK, Dagan R, Bailleux F, Haikala R, Ekstrom N, Eskola J, Yaich M, Kayhty H Functional activity of antibodies after immunization of Finnish and Israeli infants with an 11-valent pneumococcal conjugate vaccine. Vaccine 2005 Aug 20;. PMID: 16118028 [PubMed - Publisher]

Rose MA, Schubert R, Strnad N, Zielen S Priming of immunological memory by pneumococcal conjugate vaccine in children unresponsive to 23-valent polysaccharide pneumococcal vaccine. Clin Diagn Lab Immunol 2005 Oct;12(10):1216-22. PMID: 16210486 [PubMed - In-Data-Review]

Immunogenicity and Safety of a Combination Pneumococcal-Meningococcal Vaccine in Infants: A Randomized Controlled Trial Jim P. Buttery; Anna Riddell; Jodie McVernon; Tracey Chantler; Laura Lane; Jane Bowen-Morris; Linda Diggle; Rhonwen Morris; Anthony Harnden; Steven Lockhart; Andrew J. Pollard; Keith Cartwright; E. Richard Moxon. JAMA. 2005;293:1751-1758.  ABSTRACT | FULL TEXT | PDF 

Assessment of antibody response elicited by a 7-valent pneumococcal conjugate vaccine in pediatric human immunodeficiency virus infection. Clin Diagn Lab Immunol. 2005 Jan;12(1):165-70. Tarrago D, Casal J, Ruiz-Contreras J, Ramos JT, Rojo P, Snippe H, Jansen WT; Spanish Network Pneumococcus Study Group.
PMID: 15643002 [PubMed - in process]

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Yersinia Pestis

Protein Microarray for Profiling Antibody Responses to Yersinia ...
A protein microarray representing 149 Yersinia pestis proteins was ... The F1 or
V single-subunit vaccine and the F1 plus V combination vaccine have been ...

Anti-LcrV Antibody Inhibits Delivery of Yops by Yersinia pestis KIM5 by Directly Promoting Phagocytosis. Cowan C, Philipovskiy AV, Wulff-Strobel CR, Ye Z, Straley SC
Infect Immun 2005 Sep;73(9):6127-37. PMID: 16113334 [PubMed - In-Data-Review] RelatedArticles

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PneumoADIP  At least one child dies of pneumococcal disease every minute, according to current World Health Organization Estimates.  To combat this, GAVI and the Vaccine Fund established PneumoADIP at Johns Hopkins Bloomberg School of Public Health.

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Assay and Cytometry Methodologies

Also, articles on:

Escherichia coli

Group B Streptococcus

Neisseria meningitidis

Staphylococcus Aureus

Streptococcus pneumoniae

Yersinia Pestis

 
 
             
 

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